If I create a 3d file in Fusion 360 and try to run it on Carbide Create (not Pro) will it work?
No.
A 3D file needs a 3D CAM program, you will need Carbide Create Pro, MeshCAM, or some other 3D CAM solution.
That said, it will work to create the toolpaths in Fusion 360, write them out as G-code, then load that into Carbide Motion and send it to the machine to cut.
You can use Fusion to generate the tool paths.
Use the Post from Fusion for the Carbide machine.
You would read the resulting Gcode into Carbide Motion to drive the machine.