Yes, but the pulses will be done in terms of microsteps which map to # of steps/mm, so 1/40th of an mm for X and Y when using GT2 2mm pitch belts and 20 tooth count pulleys on an SO2 or later.
That said, 1 inch (25.4mm) will result in 1,016 micro-steps, so should be an exact and replicable motion.
Okay, can’t resist carrying this to the logical conclusion, so:
1/2" == 508 micro-steps
1/4" == 254 micro-steps
1/8" == 127 micro-steps
and one can’t hold an odd sixteenth of an inch w/o being off by half a micro-step (1/40th of an mm)
Continuing w/ metric we get:
0.5mm == 20 micro-steps
0.25mm == 10 micro-steps
0.125mm == 5 micro-steps
and again, one can’t hold an odd sixteenth of an mm w/o being off by half a micro-step, but given the rarity of specifying thus, metric gets the nod here.
Are pulleys w/ 16 teeth available? They’d move a bit slower, but the grid would better match up w/ dimensions, or should we go the other way and try pulleys w/ 32 teeth?